ABSTRACT
BACKGROUND According to the last 2023 Monkeypox (Mpox) Outbreak Global Map from the Centres for Disease Control and Prevention (CDC), more than 100 countries with no Mpox infection report cases. Brazil stands out in this group and is the second country with the highest number of cases in the last outbreak. OBJECTIVE To contribute to knowledge of the virus infection effects in a cellular model, which is important for diagnosis infections not yet included in a provider´s differential diagnosis and for developing viral inhibition strategies. METHODS We describe a virus isolation protocol for a human clinical sample from a patient from Brazil, the viral growth in a cell model through plaque forming units (PFU) assay, reverse transcriptase polymerase chain reaction (RT-PCR) and transmission electron microscopy (TEM). FINDINGS We follow the viral isolation in Vero cell culture from a Mpox positive clinically diagnosed sample and show the infection effects on cellular structures using a TEM. MAIN CONCLUSIONS Understanding the impact of viral growth on cellular structures and its replication kinetics may offer better strategies for the development of new drugs with antiviral properties.
ABSTRACT
The influenza A(H3N2) virus has circulated worldwide for almost five decades and is the dominant subtype in most seasonal influenza epidemics, as occurred in the 2014 season in South America. In this study we evaluate five whole genome sequences of influenza A(H3N2) viruses detected in patients with mild illness collected from January-March 2014. To sequence the genomes, a new generation sequencing (NGS) protocol was performed using the Ion Torrent PGM platform. In addition to analysing the common genes, haemagglutinin, neuraminidase and matrix, our work also comprised internal genes. This was the first report of a whole genome analysis with Brazilian influenza A(H3N2) samples. Considerable amino acid variability was encountered in all gene segments, demonstrating the importance of studying the internal genes. NGS of whole genomes in this study will facilitate deeper virus characterisation, contributing to the improvement of influenza strain surveillance in Brazil.
Subject(s)
Adult , Aged , Female , Humans , Male , Middle Aged , Glucocorticoids/administration & dosage , Hepatitis B, Chronic/drug therapy , Prednisolone/administration & dosage , Severity of Illness Index , Acute Disease , Hepatitis B, Chronic/mortality , Hepatitis B, Chronic/pathology , Immunosuppressive Agents/administration & dosage , Necrosis , Treatment OutcomeABSTRACT
Objetivo: Determinar a frequência de disfunção tireoidiana autoimune (DAT) em pacientes com diabetes mellitus tipo 1 (DM1) acompanhados pelo Serviço de Endocrinologia Pediátrica do Hospital da Santa Casa de Misericórdia de Vitória-ES (HSC). Materiais e métodos: Foi realizado um estudo de coorte transversal nos quais 38 prontuários médicos de pacientes de ambos os sexos, com diagnóstico de DM1, foram revisados e deles coletados dados referentes à função tireoidiana, como se deu a descoberta do diabetes, história familiar de tireoidopatias, dosagens TSH, T4L, anticorpos antireoidianos e dose diária de insulina. Resultados: Dos pacientes analisados, 18,4% tiveram o diagnóstico de hipofunção tireoidiana corroborando com estudos brasileiros realizados. Conclusão: Devido à elevada prevalência de doença autoimune da tireoide em diabéticos, é justificável uma investigação rotineira da função tireoidiana nesses pacientes.